FITC labelled antibodies for IHC

From:Gayle Callis

You could try an antiFITC antibody, DAKO has an excellent one but it is
rabbit antiFITC.  However, if your primary is rabbit host, that is not a
good idea.  It works at much lower concentration if you use the rabbit
antiFITC rather than Rb antiFITC-HRP. You would have to detect rabbitxFITC
with a donkey antirRb or Swine antiRb, etc. DAKO's Envision system can also
be used. Chris van der Loos does this with great skill for FITC conjugated

Greg wrote: I'm wondering if any of you fellow Vet Medicine types know of a 
source of antibodies (Poly- or Monoclonal) to Porcine Cirocovirus 
and Bovine Respiratoty Syncytial Virus (BRSV)? 

I see our friends at VMRD have Direct FA conjugates to these; 
which leads me to my next question: what is to be expected from 
staining formalin-fixed paraffin embedded tissue sections with 
direct FA ab's? (I think I'll re-post this particular question to the list 
with the subject: "Direct FA on FFPE", to get a wider response. My 
apologies in advance to those of you who end up opening both 

I seem to remember trying this at one time (when I worked in a 
virology lab) and had good results. Does it really depend on the 
antigen in question and how it responds to fixation and processing?

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)


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