Cell Culture for IHC
I have been asked to do an immunohistochemistry stain (West Nile Virus) on cell culture. I usually on worked with paraffin blocks and smears. I'm not even sure of the questions to ask, but here it goes....
In growing the cells, can I used glass slides (I don't have any plastic)?
Are the glass slides plain or charged?
How do I fix the cells? (I know if they're not fixed in formalin there will be no antigen retrieval.)
Anything special I need to know before the jump such as potential problems or background staining?
Thanks in advance,
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