Cell Culture for IHC

From:bliven.laura@marshfieldclinic.org


I have been asked to do an immunohistochemistry stain (West Nile Virus) on cell culture.  I usually on worked with paraffin blocks and smears.  I'm not even sure of the questions to ask, but here it goes....

In growing the cells, can I used glass slides (I don't have any plastic)?
Are the glass slides plain or charged?
How do I fix the cells?  (I know if they're not fixed in formalin there will be no antigen retrieval.)
Anything special I need to know before the jump such as potential problems or background staining?

Thanks in advance,
Laura Bliven
Marshfield Laboratories
blivenl@marshfieldclinic.org
(715)387-7810  




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