Boric Acid-Borate Buffer (pH 7.6)

Stock Solution A: Boric Acid   1.24gm/100ml DI water
Stock Solution B: Sodium Borate 1.9gm/100ml DI water.

Solution A.......................85 ml
Solution B.......................15 ml

Mix solution A and B.  Stable at room temperature

Supplies

Boric Acid(H3BO3)  Mallinckrodt  Cat#2549
Sodium Borate (Na2B4O7 X 10H2O) J.T. Baker Cat#3570-01

Use for 1 minute immediately after HCL step.

---- Original Message ----
From: dwalker@selway.umt.edu
To: histonet@pathology.swmed.edu
Subject: RE: Borax treatment
Date: Mon, 03 Feb 2003 12:44:24 -0700

>I am posting this for a Graduate Student:
>Dear Histonetters-
>I am doing a double-labeling immunohistochemistry protocol for smooth
>muscle
>and BrdU.  The method calls for an HCl treatment to denature the DNA
>followed by a Borax treatment to stabilize the DNA.  Does anyone have
>a
>Borax treatment protocol, including recipes?  Thank you in advance
>for your
>responses.
>
>Regards,
>Taren Grass
>
>Dave
>
>