alcian blue pH 0.4
Is anyone else using Lee Luna's Alcian Blue pH 0.4 method for
staining strongly acidic sulfated mucopolysaccharides? If you are,
what is the pH of your phosphate-HCl solution and your 1% Alcian blue
solution?
The formula for each is:
Phosphate/HCl
conc. Hydrochloric Acid 42.0 ml.
sodium phosphate,
monobasic 13.8 gm.
Deionized water to make 1000ml.
1% Alcian Blue
Alcian Blue
8GX 2.5
gm.
Phosphate/HCl solution 250 ml.
We use the stain as part of our mast cell panel for animal tissues and it
works very well. We're just curious because the pH of our solutions
are normally higher than 0.4. The pH of our solutions are usually
around 0.8 or 0.9.
Any thoughts and comments are greatly
appreciated-
thank you,
Marlene
=====
Marlene C. Nardi
Technical Services Supervisor
Histology Laboratory
New York State College of Veterinary Medicine
Cornell University
Department of Biomedical Sciences
S2-124 Schurman Hall
Ithaca, NY 14853
mailto:mcc12@cornell.edu
ph. 607-253-3314 or 607-253-3303
FAX 607-253-3357
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