alcian blue pH 0.4

From:Marlene Nardi

Is anyone else using Lee Luna's Alcian Blue pH 0.4 method for staining strongly acidic sulfated mucopolysaccharides?  If you are, what is the pH of your phosphate-HCl solution and your 1% Alcian blue solution?
The formula for each is:

conc. Hydrochloric Acid 42.0 ml.
sodium phosphate, monobasic     13.8 gm.
Deionized water to make 1000ml.

1% Alcian Blue
Alcian Blue 8GX         2.5 gm.
Phosphate/HCl solution  250 ml.

We use the stain as part of our mast cell panel for animal tissues and it works very well.  We're just curious because the pH of our solutions are normally higher than 0.4.  The pH of our solutions are usually around 0.8 or 0.9. 
Any thoughts and comments are greatly appreciated-      
thank you,

Marlene C. Nardi
Technical Services Supervisor
Histology Laboratory
New York State College of Veterinary Medicine
Cornell University
Department of Biomedical Sciences
S2-124 Schurman Hall
Ithaca, NY 14853
ph. 607-253-3314 or 607-253-3303
FAX  607-253-3357

<< Previous Message | Next Message >>