alcian blue pH 0.4
Is anyone else using Lee Luna's Alcian Blue pH 0.4 method for
staining strongly acidic sulfated mucopolysaccharides? If you are,
what is the pH of your phosphate-HCl solution and your 1% Alcian blue
The formula for each is:
conc. Hydrochloric Acid 42.0 ml.
monobasic 13.8 gm.
Deionized water to make 1000ml.
1% Alcian Blue
Phosphate/HCl solution 250 ml.
We use the stain as part of our mast cell panel for animal tissues and it
works very well. We're just curious because the pH of our solutions
are normally higher than 0.4. The pH of our solutions are usually
around 0.8 or 0.9.
Any thoughts and comments are greatly
Marlene C. Nardi
Technical Services Supervisor
New York State College of Veterinary Medicine
Department of Biomedical Sciences
S2-124 Schurman Hall
Ithaca, NY 14853
ph. 607-253-3314 or 607-253-3303
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