May I suggest you try a different HIER than lead thiocyanate. You are going
to
have a disposal problem with this - lead. It will most likely have to be
hauled away by a licensed chemical disposal company, and this will
cost money.

Plus, when you heat this solution, the thio (sulfur) part splits off from
the cyanate part. And who wants to be breathing in vapors of
lead, sulfur and a cyanate.

This was the original solution recommended and originally used. But
the original article didn't rate it much higher than citrate buffer, if
I remember which was other 2nd place solution. Since the
original article 10+ years ago, there have been many other HIER
solutions and heating methods devised.

Maybe, when our wonderful Histonetters let you know which
antibody they use that is specific for neutrophils, they could also
let you know which HIER buffer they use and at what pH.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

----- Original Message -----
From: "Lab Histopathology" 
To: 
Sent: Wednesday, February 27, 2002 5:36 PM
Subject: lead thiocynate


> Dear Histonetters,
>
> Does anyone out there have any experience (good or bad) with lead
thiocyanate for antigen retrieval for neutrophils?
>
> We have a researcher who is wanting to try this.
>
> Also,  she needs to know of any antibodies specific for neutrophils.
Help?
>
> Thank you in advance,
>
> Rosemary Vollmar
> Texas A&M University
>  979  845-5149
>  rvollmar@cvm.tamu.edu
>