I'm using hematoxylin, doing a fairly light staining,
for counterstain with our genomic ISH protocol. No problems -
the signal is strong enough that the less-than optimal color
contrast doesn't matter.

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   Mikael Niku             URL: www.helsinki.fi/~mniku/
   University of Helsinki  Dept. Basic Veterinary Sciences
       - Mitäkö mieltä olen länsimaisesta sivistyksestä?
         Minusta se olisi erinomainen ajatus!
                                              - Gandhi
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----- Original Message -----
From: "Gayle Callis" 
To: 
Sent: Monday, February 25, 2002 7:29 PM
Subject: counterstaining with ISH


> Are people counterstaining sections after ISH using nonradioactive method
> with BCIP/NBT chromogen?  If so what do you prefer?
>
>
> Gayle Callis
> MT,HT,HTL(ASCP)
> Histopathology Supervisor
> Veterinary Molecular Biology - Marsh Lab
> Montana State University - Bozeman
> 19th and Lincoln St
> Bozeman MT 59717-3610
>
> 406 994-6367
> 406 994-4303 (FAX)
>
>
>