Re: counterstaining with ISH
|From:||firstname.lastname@example.org (Hermina Borgerink)|
I don't usually counterstain after ISH but when I do, I use 0.1% aqueous
light green. I have also tried eosin but was not happy with the results.
Some people use methyl green.
Gayle Callis wrote:
> Are people counterstaining sections after ISH using nonradioactive method
> with BCIP/NBT chromogen? If so what do you prefer?
> Gayle Callis
> Histopathology Supervisor
> Veterinary Molecular Biology - Marsh Lab
> Montana State University - Bozeman
> 19th and Lincoln St
> Bozeman MT 59717-3610
> 406 994-6367
> 406 994-4303 (FAX)
Hermina Borgerink, BA, HTL, HT(ASCP)IHQ
Department of Pathology
Wake Forest University School of Medicine
Winston-Salem, N.C. 27157
PH (336) 716-1538
Fax (336) 716-1515
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