Re: counterstaining with ISH

From:hborgeri@wfubmc.edu (Hermina Borgerink)

Hi Gayle,

I don't usually counterstain after ISH but when I do, I use 0.1% aqueous
light green.  I have also tried eosin but was not happy with the results.
Some people use methyl green.

Hermina

Gayle Callis wrote:

> Are people counterstaining sections after ISH using nonradioactive method
> with BCIP/NBT chromogen?  If so what do you prefer?
>
> Gayle Callis
> MT,HT,HTL(ASCP)
> Histopathology Supervisor
> Veterinary Molecular Biology - Marsh Lab
> Montana State University - Bozeman
> 19th and Lincoln St
> Bozeman MT 59717-3610
>
> 406 994-6367
> 406 994-4303 (FAX)

--
Hermina Borgerink, BA, HTL, HT(ASCP)IHQ
Department of Pathology
Wake Forest University School of Medicine
Winston-Salem, N.C. 27157
PH (336) 716-1538
Fax (336) 716-1515






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