Re: bone marrow decalcification
Steven,
there have been some studies and papers written demonstrating that formic acid
is a very good decal reagent for IHC. does something change with using the MW.
Patsy
Steven Slap wrote:
> Hi HistoNetters
>
> Roxanne Soto asked about microwave procedures for bone marrow
> decalcification. What I would recommend would depend upon the outcome
> desired- if immunohistochemistry is the primary aim, I would use EDTA; if
> speed is the main objective, I would use one of the formic acid methods.
>
> 1) EDTA at 50°C for 60-90 minutes
>
> 2) 5% formic acid at 50°C for 10-20 minutes
>
> 3) 5% formic/5% hydrochloric acid at 50°C for 5-10 minutes
>
> All times cited are times at temperature (not counting ramp up times). In
> all cases, decalcification should be checked at regular intervals by opening
> the door of the microwave (or pausing the program) and feeling the biopsy
> with a needle.
>
> If higher concentrations of acid are used, the temperature should be lowered
> to 37°C.
>
> best regards,
> Steven Slap
>
> **********************************************
> Marketing Manager/Microwave Product Specialist
> Hacker Instruments & Industries, Inc.
> http://www.hackerinstruments.com
> **********************************************
> --
<< Previous Message | Next Message >>