Re: bone marrow decalcification

From:rueggp

Steven,
there have been some studies and papers written demonstrating that formic acid
is a very good decal reagent for IHC.  does something change with using the MW.
Patsy

Steven Slap wrote:

> Hi HistoNetters
>
> Roxanne Soto asked about microwave procedures for bone marrow
> decalcification.  What I would recommend would depend upon the outcome
> desired-  if immunohistochemistry is the primary aim, I would use EDTA;  if
> speed is the main objective, I would use one of the formic acid methods.
>
> 1)  EDTA at 50C for 60-90 minutes
>
> 2)  5% formic acid at 50C for 10-20 minutes
>
> 3)  5% formic/5% hydrochloric acid at 50C for 5-10 minutes
>
> All times cited are times at temperature (not counting ramp up times).  In
> all cases, decalcification should be checked at regular intervals by opening
> the door of the microwave (or pausing the program) and feeling the biopsy
> with a needle.
>
> If higher concentrations of acid are used, the temperature should be lowered
> to 37C.
>
> best regards,
> Steven Slap
>
> **********************************************
> Marketing Manager/Microwave Product Specialist
> Hacker Instruments & Industries, Inc.
> http://www.hackerinstruments.com
> **********************************************
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