Re: aqueous mounting media
I am not sure that there is a really effective aqueous mountant that will
retain methylene blue. The reason is that, as you pointed out, in aqueous
media it leaches out, in most non aqueous media it becomes reduced to
colorless products ( but xcan be reconstituted on exposure to air)..
One solution that used to be common for metachromatic studies with methylene
blue (and toluidine blue) was to allow the section to dry and to store dry.
When you needed to examine the section you added the aqueous mounting media.
This process could be repeated several times.
You might also consider using toluidine blue as it is much more resistant to
"Bartlett, Jeanine" wrote:
> Hi Histonetters!
> Can anyone out there suggest an aqueous mounting media that can be used on
> sections when methylene blue has been used as the counterstain? I have
> tried several but they all leach the blue out of the section.
> Vendors welcome to reply!
> Jeanine Bartlett, HT(ASCP)
> Centers for Disease Control
> Infectious Disease Pathology Activity
> 1600 Clifton Rd., N.E. MS-G32
> Atlanta, GA 30333
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