Don't know if this will help, but there is a NSH teleconference on
cytochemical detection of apoptotic cell death on May 15, 1-2 pm
Eastern time zone.
For more information, go to
Or contact NSH at 301-262-6221
May 15, 20027
Cytochemical Detection of Apoptotic Cell Death
The detection of cell death has recently gained central importance in the
study of various diseases, such as cancer and neurodegenerative diseases
(e.g. Alzheimer's and Parkinson's diseases). Cells undergo death by two
major mechanisms: necrosis, in which primary damage to the metabolic or
membrane integrity of the cell occurs, or apoptosis, which is an internal
suicide program contained in all cells. This presentation will summarize
the knowledge about apoptotic pathways, and will discuss the
immunohistochemistry of caspase-cleaved epitope antibodies in paraffin
sections of tissues.
Presenter: Mark C. Willingham, M.D.
Wake Forest University School of Medicine
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
(and NSH Teleconference Coordinator)
----- Original Message -----
Sent: Thursday, February 21, 2002 3:27 PM
> I am going to incorporate apoptosis assays into our routine lab procedures
> and would like some input into which detection methods people have found
> they like the best and since we always made up our own solutions I would
> also like to know which kits if any that people use routinely. I need
> flexible visualization because of the preferences of the different
> investigators and will be viewing paraformaldehyde fixed paraffin embedded
> tissues and cell supensions mouse tumors and bone marrow smears.......too
> much info? not enough? let me know.
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