Re: adipocyte and osteoblast markers


There is an antibody to TRAP which will stain osteoblasts.  The acid phosphatase enzyme method for osteoblasts does not work well on ffpe samples in my experience.
It seems that fat cells are always staining in just about any IHC I do, as well, should be easy to mark them.
Patsy Ruegg

"Johnson, Teri" wrote:

> I've searched Abcam's site for markers for adipocytes and osteoblasts.  A post-doc is interested in specific markers for these two cell types, or any special histochemical stains as well.  We are interested in demonstrating these in formalin/paraformaldehyde fixed, paraffin embedded tissues.  Therefore the usual fat stains would not be appropriate.
> Interestingly, he showed me pictures of what he called specific staining of the area surrounding fat globules (paraffin section) with an antibody, and it looked strikingly similar to what I've seen before as a non-specific finding (maybe S-100?).  At any rate, any help is appreciated.
> Teri Johnson
> Manager Histology Core Facility
> Stowers Institute for Medical Research
> 1000 E. 50th St.
> Kansas City, Missouri  64110

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