Re: Tree Shrew
Tree Shrews are mammals closely related to
primates. (Tupia Glis) The protein sequences that we have are very
similar (95 % or more) to human. We are one of 3 labs in the US breeding
them.
We haven't done any IHC to speak of but were thinking of doing some
frozen sections of Sclera in the future. I don't really know the
specifics of fixation but Pam tried some sections that were fixed. I've
been trying comercial antibodies (human antibodies known to work in other
species) in western blots. An ideal antibody could be used for either
technique. Proteins of interest are extracellular matrix proteins (TIMP I
& II, MMP 1-3, 26, more...). We know these proteins are in sclera and
expressed at detectable levels. I feel that my extraction and western
blotting techniques are working. I had success with a human Actin
antibody and with a TIMP 2 human polyclonal that is no longer avalable.
JOEL
At 12:03 PM 2/26/02 -0700, you wrote:
Being a rodent, similar to
mouse??? What kind of IHC is being done, more
specifics on tissue handling, fixed versus frozen sections, etc, etc.
would
help.
At 10:48 AM 2/26/02 -0600, you wrote:
>Hi All!
>By chance, is anyone here working with IHC on Tree Shrew? I've got a
bud
>next door who is having major probs...Any help would be greatly
>appreciated...regards...
>
>tammy
>
>Tammy M. Bailey
>University of Alabama Birmingham
>Histology Module
>Vision Science Research Center
>205-934-3074-lab
>205-410-8029-cell
>tbailey@uab.edu
>
>
>
>
Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
Tammy M. Bailey
University of Alabama Birmingham
Histology Module
Vision Science Research Center
205-934-3074-lab
205-410-8029-cell
tbailey@uab.edu
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