Re: Tissue shrinkage
TOMANDBOB@aol.com wrote:
> Histonetters: Does anyone have any ideas/articles/ references on how to prevent or minimize tissue shrinkage for specimens going into a non-aqueous plastic polymer such as Epon or Spurr? Or is this the impossible dream?
> Thanks,
> Tom Kuwahara
> Senior Immunohistochemist
> Resolution Sciences Corporation
I have always been told/thought that shrinkage of tissues in epoxy resins is much less than in paraffin sections. Proper fixation and subsequent processing are your best "defense".
Geoff
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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