Re: Tissue shrinkage

From:Geoff McAuliffe wrote:

> Histonetters:  Does anyone have any ideas/articles/ references on how to prevent or minimize tissue shrinkage for specimens going into a non-aqueous plastic polymer such as Epon or Spurr?  Or is this the impossible dream?
> Thanks,
> Tom Kuwahara
> Senior Immunohistochemist
> Resolution Sciences Corporation

I have always been told/thought that shrinkage of tissues in epoxy resins is much less than in paraffin sections. Proper fixation and subsequent processing are your best "defense".

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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