Re: Spin on IHC buffers

From:Amos Brooks

    Part of the senseless babbling I was referring to involves osmotic
balance but it didn't make much sense the way I described it. I'm glad that
I was on the same wavelength though.
    The tissue is formalin fixed and paraffin embedded. So does that prevent
the deleterious effects? If it does then people are wasting a lot of money
on buffer. I for one am not quite ready to take such a chance but it bears

----- Original Message -----
From: "Monson, Frederick C." 
To: "'Amos Brooks'" 
Cc: "'List-HistoPath'" 
Sent: Thursday, February 21, 2002 4:26 PM
Subject: RE: Spin on IHC buffers

> Hi Amos,
> One of my favorite sub-subjects.  Colligative properties of
> solutions.  I think the words you want are "osmotic pressure".  If
> are fresh-frozen and thick enough to house unsectioned cells, then just as
> in a suspension of RBC's, diHOH will 'fill them to bursting', because of
> "osmosis".   Same thing to the unfixed "ground substance" of connective
> tissue.  Maybe I should issue forth on "Chromatin" and "Metachromasia".
> yes!  My alcohol of choice for dehydrating tissues stained with
> metachromatic dyes is Tertiary ButAnal ("TBA" - Now why would anyone think
> to  give the suffix of that word so much prominence?)  In any case, with
> TBA, the metachromasia assuredly survives.  I've had some in Azure B (DNA
> vs. RNA) mounted in Damar for 20 years and still see azure and purple.
> Get those people free tickets to see "Osmosis Jones".
> By the way, if the tissue is fixed, diHOH probably won't have any
> deleterious effect on the results.  I mean, after we use the magic tap
> on the Schiff's Reagent, don't we rinse in diHOH before we enter the
> alcohols.  No?  For Heaven's sake, why not!!!???!!!  Wouldn't we
> the alcohol dilutions we made with diHOH?
> Has anyone ever determined why the tap water in Duluth works better
> on Schiff's than that in Minneapolis?  I never did hear how that debate
> resolved.  I missed most of it by becoming electron microscopic.
> Well, Amos, I am taking tomorrow off, because I need to replace a
> windshield wiper motor.  I'm starting at the exhaust pipe and I'm going to
> work my way forward until I find that little bugger.  Then, after lunch,
> while I revel in my mechanical skills, I'm going to watch the US team beat
> the Russians in ice hockey and, hopefully, the Canadians beat the
> Liliputians.
> My best wishes for all to have a peaceful weekend, especially our
> neighbors who are sailing with the Navy, flying with the Air Force
> (robotically with the Army) and sand flea hunting with the Marines.
> Fred Monson
> Frederick C. Monson, PhD
> The best research
> Center for Advanced Scientific Imaging
> occurs before work
> West Chester University
> at the bench.
> West Chester, Pennsylvania, USA, 19383
> 610-738-0437

<< Previous Message | Next Message >>