Re: Spin on IHC buffers

From:Amos Brooks

Oh yeah,
    I forgot ... the dH2O has tween20 in it. (Could that be the reason for
the blown up frozen sections?)
Amos

----- Original Message -----
From: "Gayle Callis" 
To: "Amos Brooks" ; 
Sent: Thursday, February 21, 2002 12:31 PM
Subject: Re: Spin on IHC buffers


> It might work on paraffin sections???  HOWEVER, I have made enough
mistakes
> by grabbing the wrong rinse bottle containing deionized water for rinsing
> and BLEW APART MY FROZEN SECTIONS INTO PIECES OF UNREADABLE JUNK!
>
>
> At 07:26 PM 2/20/02 -0500, you wrote:
> >Hi,
> >    I have heard of a lab recently that does not use buffer for their IHC
> >staining anymore. They have switched to deionized water. Now, of course I
> >know not to do this but I was hard pressed to put into words why. I would
be
> >thrilled to hear a more eloquent explanation than what I am babbling on
> >about.
> >Amos Brooks
> >
> >
> >
> >





<< Previous Message | Next Message >>