Re: Osmium Tetroxide

From:Dimitri Scholz

Re: Osmium Tetroxide
Hi Anila,

You are correct, osmium tetroxide does stabilise lipids in the myelin sheaths and therefore gives much contraster pictures. It does also good especially for nerve tissue and  for  toluidine blue staining. But be careful, the stuff is dangerous. If you smell it, you got already too much. Buy only dissolved 4% solution of it, dont try to dissolve the powder. Work only in fume cupboard!

Good luck

Dimitri Scholz
--
_________________________________________
Dr. Dimitri Scholz
Max-Planck-Institute
Dept. of Exp. Cardiology
Beneke-Str.2
D-61231 Bad Nauheim
Germany
voice    ++49 6032 705-403
Fax      ++49 6032 705-419
Email    d.scholz@kerckhoff.mpg.de

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