Re: Levels on biopsies

From:=?iso-8859-1?q?Tony=20Henwood?=

Mike,
This is from our manual. It might help.

Tony

Definitions
Sometimes a pathologist may request more sections to
be cut from a block. 
The following definitions are a guide only and will
depend on the type and thickness of the tissue in the
block.

RECUT:    
X This may be requested because of a poor initial
section (eg not deep enough, poor staining, lifting)
X Cut a better section (deeper or use an adhesive if
required).

SERIAL SECTIONS:
X Cut consecutive sections WITHOUT trimming.

LEVELS:   
X Trim in 30um and then section

DEEPER:   
X Trim in 100um and then section.


REMEMBER it is always better to not trim too far than
to trim it all away.

If in doubt cut levels anyway.


 --- Mike Black  wrote: > What are
your definitions of levels (25%,50%,75% ?)
> when cutting biopsies?? How many slides and levels
> are appropriate for a biopsy??
> 
> Mike Black
> Manager, Laboratory Operations
> Covenant Medical Center
> 3615 19th Street
> Lubbock, Texas 79410
> (806) 725-0037
> 
>  

=====
Tony Henwood JP BAppSc GradDipSysAnaly CT(ASC)
Laboratory Manager
Histopathology Department
The Childrens Hospital at Westmead
Locked Bag 4001
WESTMEAD, 2145
AUSTRALIA

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