Re: Info on "RHODANINE"
The stain is simple.
Stock Rhodanine saturated
sol. 0.5% Sodium Borax
5 p-dimethylaminobenzylidene Rhodanine 0.2
gm. Sodium Borax 0.5gm
Absolute ethanol 100.0
ml Distilled water 100.0 ml
sol. 3.0ml Shake stock
Distilled water 100.0 ml
The Rhodanine we are using was obtained from Eastman Kodak many years ago
the cat no. was 2748
1 Deparaffinize and hydrate to distilled water
2 Place slides in a plastic coplin jar containing the working Rhodanine
solution. Microwave in a 1200 watt microwave on 50% power for 30 seconds.
Allow slides to remain in the solution as it cools down , about 5 minuttes.
3 Wash well in several changes of water
4 Mayer's hematoxylin for 5 minutes
5 Wash well in running tap water
6 Rinse slides in 0.5% sodium Borax
7. Rinse well in distilled water
8. Dehydrate, clear and mount
it is best not to let the slides stand in xylene for a prolonged period,
any carryover of ethanol will fade the stain
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