Re: Immuno Buffers

From:"John C. Dennis"


I make up 1 L solutions of TRIS at pH whatever and 0.25 or 0.5 M EDTA
solutions.  Autoclave 20 minutes at 121 deg C (250 deg F) and keep them on
the shelf at RT.  Use those stocks to make up the volume of fresh
buffer that you'll need for whatever job is at hand.  Be sure not to
stick anything into the stock
bottles when you want a volume.  Always decant as you would with any good

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849

On Wed, 20 Feb 2002, Maria Mercier wrote:

> Hi all
> Can one make up Tris and EDTA buffers in bulk and use
> as needed or is it better to make them up fresh?At
> what temp should they be kept and what is their shelf
> life?
> Thanks in advance for your help.
> Maria
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