Re: HistoGel

From:"David A. Reynolds"

I don't know if this pertains to your situation, but when I used HistoGel on cytology cell block preps I would spin down the specimen in a centrifuge, draw off the supernatant and replace it with HistoGel.  When the gel hardened we would end up with a pointed gel blob with the specimen at the point.  So if the specimen was visible or not, we always knew where it was in the gel.  I have never seen the gel come out of the processor, although when our blocks were on ice it would turn white.

Dave Reynolds, HT(ASCP)
University of Washington Medical Center
Seattle, WA

On Mon, 25 Feb 2002, John Spair wrote:

> Does anyone, or has anyone used HistoGel from Richard Allan for their
> biopsies or cell blocks???  When we first tried out the samples the gel came
> through clear or with slight pink tinge after processing.  This was great,
> as you could see the specimen inside - now the come through white after
> processing and you can't see anything when you're cutting the block.  I've
> tried different lot numbers to no avail, and I haven't change my processing
> routine for probably 5 or 6 years now.  I anxiously await to hear of other
> users and their experience.  Thank you.
> John Spair, Manager 
> Pathology Services 
> MultiCare Health System 
> 253-403-6090 

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