Re: Help with a coverslipping problem
The slides must not be completely
dehydrated prior to coverslipping. It could also be that the slides sat out of
xylene long enough to absorb atmospheric humidity which could also be a culprit.
My advise is to rotate the alcohols more and use XYLENE cause darnit it's just
----- Original Message -----
Sent: Thursday, February 28, 2002 11:17
Subject: Help with a coverslipping
I just spoke to a very frustrated lady who needs help with a
coverslipping problem. She has a Leica coverslipper (which is
functioning well), but she recently converted from using Richard Allan Clear
Rite and compatible medium to xylene and StatLab's Acrymount. She purged
the instrument but she is seeing cloudiness around the perimeter of
the coverslip, which seems to be getting increasingly worse.
It sounds like something was missed during the purge. Do any of the
Leica coverslipper users have any suggestions? I will forward your
suggestions to her or you can email her directly at firstname.lastname@example.org
Histology Consulting Services, Inc.
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