Thanks for the input, I never do formalin fixed paraffin embedded
immunowork, so I stand corrected. Actually, Barb Wright was a source but
also Vector.    

I had a wonderful holiday weekend attending Winter Olympics in SLC, seeing
Ohno short track race,  120m hill team ski jumping, medals ceremony and
concert, and biathalon - all in wonderful springlike skiing conditions, a
picnic in the snow.  Contrary to many reports on security and long lines,
if you had an event ticket, security checks were 10 min at most in line
plus all people were absolutely wonderful and patient. It was an
international celebration!  




At 08:47 AM 2/21/02 -0800, you wrote:
>hi gayle,
>just thought i'd impart a couple of comments.  correct me if i'm wrong, but
>barb wright was the one who passed this little gem on to you?  i was the one
>who put this protocol in place at genetech.  after having lots of problems
>quenching eosiniphilic peroxidase in a mouse asthma model, i did a lit.
>search and found the protocol.  we tried it, liked it and used it for all
>frozens (not just minimally fixed).  i found that it worked fine for
>paraffin sections too.....but we used the KPL H2O2 block (which i later
>found out is periodic acid) which worked as well and took much less time and
>since we didn't need to be quite as gentle.....it made more sense to use the
>KPL stuff.
>hope you had a nice holiday weekend!  :-)  (i managed to spend mine getting
>really sick....ugh!  :-()
>carrie
>
>Carrie Kyle-Byrne, BHS, HT(ASCP)
>Assoc. Research Scientist II
>Molecular Target Research
>
>Exelixis, Inc.
>170 Harbor Way
>P.O. Box 511
>South San Francisco
>CA 94083-0511 USA
>
>Phone: (1 650) 837-8023
>Fax: (1 650) 837-7240
>Email: ckbyrne@exelixis.com
>
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>
>----- Original Message -----
>From: "Gayle Callis" 
>To: 
>Sent: Wednesday, February 20, 2002 10:30 AM
>Subject: Glucose oxidase blocking method, a reminder on usage
>
>
>> To those who are receiving my attachment on glucose oxidase blocking for
>> endogenous peroxidase and pseudoperoxidase in frozen sections.
>>
>> This method is for MINIMALLY FIXED (acetone, acetone/alcohol) frozen
>> sections and NOT formalin fixed paraffin sections or even paraformaldehyde
>> fixed frozen sections.
>>
>> I suggest you also access the referenced publications and read about the
>> method.
>>
>> If you encounter any problems, email me
>>
>>
>> Gayle Callis
>> MT,HT,HTL(ASCP)
>> Histopathology Supervisor
>> Veterinary Molecular Biology - Marsh Lab
>> Montana State University - Bozeman
>> 19th and Lincoln St
>> Bozeman MT 59717-3610
>>
>> 406 994-6367
>> 406 994-4303 (FAX)
>>
>>
>
>
>
Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)