I only prep urine cytology here and when I came to
this lab, we were using albumin in our cytospins.
Nobody could really tell me why, so I decided to try
some preps without albumin and we actually saw more
cells on the slides without the albumin! I use plain
glass slides (no adhesive or charge).My urines are
fixed with 50% alcohol, that may make a difference. I
don't know if anything other than urines may require
the albumin. When I previously did cytoprep work (5
years ago) I didn't even own any albumin and we did
other types of specimens than just urine.
Good luck with your cyto-ventures,
Jennifer Hofecker HT(ASCP)
--- Janice Fuller wrote:
> I have a few questions: Is albumin used in the
> cytospin for any other reason than adhesion? What
> about if your already using plus or coated slides?
> I've heard for a long time that if you use an
> adhesive in your waterbath and then use plus slides
> it changes the charge and tissue falls off. I've
> not experienced this personally, but it seems that
> it would be as true for cytology as histology.
> Also, what about air drying cytospin slides for a
> Giemsa stain. Shouldn't this be a Wright Giemsa?
> Why would this be preferable to fixing in 95%
> Alcohol and pap staining? I'm trying to get a feel
> for the best way to set up a lab for cytoprep.
> Janice Fuller
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