Re: Cyclin E

From:Richard Cartun

Novocastra Laboratories, Ltd. 

R. Cartun

>>>  02/20/02 06:43PM >>>
I have a pathologist who would like me to order Cyclin E.  I have checked my 
usual sources and can't seem to find it.  Anyone out there use this antibody?

Also, the hospital I am at now has a Ventana Nexus immunostainer.  It is not 
uncommon for us to have problems every couple of weeks or so with this little 
gem. ;>))  It sounds like a lot of you do your immunos by hand.  Is this 
correct?  I used to do them by hand, used concentrated antibodies, etc.  I 
inherited this stainer when I started at this job a year ago.  I am currently 
working my way back to concentrated antibodies, rather than the Ventana 
predilutes, and at times toy with the idea of doing them by hand again.  One 
advantage that I see with the Nexus is the use of heat.  But when it goes 
down I am down 3-5 days.  This does not make for happy pathologists!   Any 
comments are welcome!

Denise Swank
Hillcrest Medical Center
Tulsa, OK

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