I have to mentally prepare myself to do nerve teasing otherwise I know I'll
just be wasting my time.I usually forewarn my colleagues so that they know I
didn't actually mean to snap at them.My advice:lots of
patience!!!!!!!!!!!!!!!!!

Isabel






Isabel de Faria Leal
Neuropathology Unit
NHLS/Wits Medical School
Johannesburg
South Africa

-----Original Message-----
From: Ronnie Houston [mailto:Ronnie.Houston@tsrh.org]
Sent: Friday, February 22, 2002 10:00 PM
To: Andrew.Shand@north-bristol.swest.nhs.uk;
histonet@pathology.swmed.edu
Subject: RE: nerve dissection/teasing


Well done Andy............................having spent days on end doing
this, and pulling every hair out of my head, I totally agree with you.

Also, opinion is very divided about what useful information you actually get
from a well-teased nerve (personally I do not believe it is worth the time
and effort). If the nerve isn't well-prepared and carefully handled, you are
wasting your time and everybody elses as the potential for artefactual
damage is great.

Ronnie


Ronnie Houston
Cytochemistry & Molecular Pathology
Texas Scottish Rite Hospital for Children
2222 Welborn Street
Dallas, TX 75219
USA
(214) 559 7744
(214) 559 7768 - fax
ronnie.houston@tsrh.org

>>>  02/22/02 07:46AM >>>
Trust me - the best method is to get somebody else to do them.

Andy Shand

-----Original Message-----
From: Maria Mercier [mailto:mariamercier@yahoo.com]
Sent: 22 February 2002 12:54
To: histonet@pathology.swmed.edu
Subject: nerve dissection/teasing


Hi all

What method are you nerve teasers using?I use the
method whereby the nerve is osmicated and then placed
in 45,60,100% glycerol.Are there any other methods?How
long do you take to tease a nerve and how many slides
per nerve do you prepare?

Thanks

Maria

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