RE: nerve dissection/teasing
Well done Andy............................having spent days on end doing this, and pulling every hair out of my head, I totally agree with you.
Also, opinion is very divided about what useful information you actually get from a well-teased nerve (personally I do not believe it is worth the time and effort). If the nerve isn't well-prepared and carefully handled, you are wasting your time and everybody elses as the potential for artefactual damage is great.
Cytochemistry & Molecular Pathology
Texas Scottish Rite Hospital for Children
2222 Welborn Street
Dallas, TX 75219
(214) 559 7744
(214) 559 7768 - fax
>>> 02/22/02 07:46AM >>>
Trust me - the best method is to get somebody else to do them.
From: Maria Mercier [mailto:firstname.lastname@example.org]
Sent: 22 February 2002 12:54
Subject: nerve dissection/teasing
What method are you nerve teasers using?I use the
method whereby the nerve is osmicated and then placed
in 45,60,100% glycerol.Are there any other methods?How
long do you take to tease a nerve and how many slides
per nerve do you prepare?
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