We used this fixative on mouse tissue for the demonstration of CD's 3,4, and
8 which were not demonstrated on formalin fixed tissue.
0.1 M Tris Buffer, pH 7.4 1000ml
Calcium acetate 0.5 g
Zinc Acetate 5.0 g
Zinc Chloride 5.0 g
Mix to dissolve. The final pH will be approximately 6.8 (6.5-7.0). Do not
readjust the pH, as this will cause the zinc to come out of solution. Small
amounts of insoluable zinc oxychloride often contaminate zinc chloride.
This may result in a very small amount of white precipitate. This will not
cause any problems but can be removed by filtering the solution.
Fix tissue for 24-48 hours (any longer and the tissue will become very
brittle) and transfer to 70% ethanol.
Beckstead JH. A Simple Technique for Preservation of Fixative-sensitive
Antigens in Paraffin-embedded Tissues: Addendum (Letter to the Editor). J
Histochem Cytochem 1994; 43:345
Infectious Disease Pathology
Centers for Disease Control
Does anyone know the following solution(fixative),used for
immunohistochemistry: 0.1MTris,0.05%calcium acetate,0.5% zinc acetate
and 0.5% ZnCl2 ph 7.4.? Problems making up the soln. goes cloudy .
Ref paper Infection and Immunity Jan 2002, p303-314, "Plasmidic
versus Insertional Cloning etc" I. Me'derle' .Can anyone help
or can I use Buffered formalin? Thanks. Marilyn
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