RE: crystallzed precipitant

From:Gayle Callis

Good point! 

This precipitate in higher alcohols also leads to clogged tubing in
automated processors and processsing run failure, a real mess!  Never
rotate a used higher percentage ie 95% alcohol down to the 70% station when
rotating solvents, always replace 70% with same concentration alcohol.    

At 12:05 PM 2/25/02 -0600, you wrote:
>You may want to check the percentage of alcohol in your alcoholic
>formalins, if it is above 70% then the formalin buffer salts are
>precipitating out in the alcohol.  
>Debbie J. Siena, HT(ASCP)QIHC 
>Histopathology Manager 
>Baylor University Medical Center 
>3500 Gaston Ave. 
>Dallas, TX 75246 
>214-820-2465 vm 
>214-820-4110 fax 
>-----Original Message-----
>From: Jeannie Heck []
>Sent: Thursday, February 21, 2002 10:08 PM
>To: Histonet
>Subject: crystallzed precipitant
>We have recently noticed a problem with a precipitant forming in our
>alcoholic formalin. We make this solution up fresh weekly. It is used in
>our tissue processor following 10% formalin we use two changes. We have
>used this technique for many years but have just recently noticed the
>crystallized precipitant. Any advice to keep the precipitant from
>forming will be appreciated.
>Jeannie Heck HT 
>Hutchinson Hospital
>  _____  
>Do You Yahoo!?
>Yahoo!   Sports - Coverage of the 2002
>Olympic Games 
Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)

<< Previous Message | Next Message >>