Ha!  That's in your processor!  In mine, I never have clogged tubing, and I
pour higher alcohols downhill routinely.  Only maintenance is changing the
light bulbs that give the heat.  Progress isn't always as kind as it should
be, and I can still process 10-15 specimens a day whenever I get really
busy.

Fred Monson

Frederick C. Monson, PhD
The best research
Center for Advanced Scientific Imaging
occurs before work
West Chester University
at the bench.
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson@wcupa.edu




> ----------
> From: 	Gayle Callis
> Sent: 	Monday, February 25, 2002 2:30 PM
> To: 	Jennings-Siena, Debbie; histonet@pathology.swmed.edu
> Subject: 	RE: crystallzed precipitant
> 
> Good point! 
> 
> This precipitate in higher alcohols also leads to clogged tubing in
> automated processors and processsing run failure, a real mess!  Never
> rotate a used higher percentage ie 95% alcohol down to the 70% station
> when
> rotating solvents, always replace 70% with same concentration alcohol.    
> 
> At 12:05 PM 2/25/02 -0600, you wrote:
> >You may want to check the percentage of alcohol in your alcoholic
> >formalins, if it is above 70% then the formalin buffer salts are
> >precipitating out in the alcohol.  
> >Debbie J. Siena, HT(ASCP)QIHC 
> >Histopathology Manager 
> >Baylor University Medical Center 
> >3500 Gaston Ave. 
> >Dallas, TX 75246 
> >214-820-2465 vm 
> >214-820-4110 fax 
> >debbiesi@bhcs.com 
> >
> >-----Original Message-----
> >From: Jeannie Heck [mailto:jeannie_heck@yahoo.com]
> >Sent: Thursday, February 21, 2002 10:08 PM
> >To: Histonet
> >Subject: crystallzed precipitant
> >
> >
> >
> >We have recently noticed a problem with a precipitant forming in our
> >alcoholic formalin. We make this solution up fresh weekly. It is used in
> >our tissue processor following 10% formalin we use two changes. We have
> >used this technique for many years but have just recently noticed the
> >crystallized precipitant. Any advice to keep the precipitant from
> >forming will be appreciated.
> >
> > 
> >
> >Jeannie Heck HT 
> >
> >Hutchinson Hospital
> >
> >
> >
> >
> >  _____  
> >
> >Do You Yahoo!?
> >Yahoo!   Sports - Coverage of the 2002
> >Olympic Games 
> >
> >
> >
> >
> Gayle Callis
> MT,HT,HTL(ASCP)
> Histopathology Supervisor
> Veterinary Molecular Biology - Marsh Lab
> Montana State University - Bozeman
> 19th and Lincoln St
> Bozeman MT 59717-3610
> 
> 406 994-6367
> 406 994-4303 (FAX)
> 
> 
>