RE: Oct-2 Antibody?Oct-2 (Ab-1) Cat# NA44 Oncogene Research Prod ucts

From:"Ward, Elizabeth"

This may help....

Oct-2 (Ab-1) Cat# NA44
The Oct proteins make up a family of transcription
factors that contain bipartite DNA-binding POU
domains, which in turn are members of the helix-turnhelix
family of DNA binding domains (1,2). Oct-1 and
Oct-2 are transcription factors that bind to the same
octamer motif, ATGCAAAT, but possess different
transcriptional activities and expression patterns (3,4).
The Oct elements are the most conspicuous regulatory
element in B cells and are found in every Ig promoter
and in most Ig enhancers (5). While the Oct-1 protein is
found ubiquitously expressed, Oct-2 (also known as
OTF2, NF-A2, and Oct-2b) appears to be restricted in
expression to B-cells, neuronal cells, and some T cells
(3,4, and Reviewed in 6). At least in B-cells, the Oct
proteins bind to DNA together with another protein
transcription factor, octamer binding factor-1 (OBF-1),
to form a ternary complex (2,7). This latter observation
suggests that DNA binding factors exhibiting cell
restricted expression may also have co-factors thereby
adding to their specificity in activity. Oct-2 undergoes
alternative splicing and multiple protein isoforms are
expressed in both B cells and neuronal cells (4).
Alternative splicing of Oct-2 generates proteins with
altered promoter selectivity (8). Neuronal cells
predominantly express two isoforms of Oct-2 that
appear to act as transcriptional repressors whereas the
isoforms predominantly seen in B cells activate
transcription (9). This dual capability of Oct-2 appears
to derive from the presence of distinct activation and
repression domains within the protein along with the
fact that Oct-2 functions as a multimer with individual
multimers composed of one or more isoforms
(Reviewed in 6). Oct-2 expression in neurons can be
induced by NGF, and the isoforms induced have
transcriptional repression activities; thus, neuronal Oct-
2 appears to be an immediate early gene whose
expression leads to transcriptional repression (10).
Clone PT1 is a mouse monoclonal antibody generated
by immunizing mice with recombinant Oct-2 (11) and
fusing the splenocytes with Sp2/0 mouse myeloma cells.
(SLWRSHNH-terminal segment of Oct-2 present in all
isoforms (4, 11).
Negative: Mouse
100 mg of purified antibody in 1.0 mL of 0.05 M
sodium phosphate buffer containing 50 % glycerol.
Store at -20C. For long term storage, aliquot into
smaller volumes and store at -20oC. Avoid multiple
freeze/thaw cycles. If stored under proper conditions,
product guaranteed until expiration date stated.
NA44 can be used in electrophoretic mobility shift
assays as described (11).
Amt Label Pretreatment Detection Positive
1 mg 35S or
IP/blot (11)
Autoradiography or
Daudi cells HeLa cells
Western Blot* 1 g/mL NA None Chemiluminescence Daudi cells HeLa cells
Frozen Sections NT
Paraffin Sections NT
#See NOTES NA: Not applicable NT: Not tested
650 Albany Street Research reagents may be ordered by mail at
Boston, MA 02118 the address shown at left, by telephone,
Tel: 617 536-2223 800 662-2616 E-mail, or fax or from your local
Fax: 617 536-9911 800 828-4871 Oncogene Research Products supplier.
E-Mail: Terms are net 30 days F.O.B.
Web Page: shipping point.
For research use only, not for use in diagnostic procedures
Oct-2 (Ab-1) Cat# NA44
1. Herr, W., et al. (1988) Genes and Dev. 2: 1513-
2. Aurora, R., and Herr, W. (1992) Mol. Cell. Biol.
12: 455-467
3. Sturm, R.A., et al. (1988) Genes Dev. 2: 1582-
4. Wirth, T., et al. (1991) Nuc. Acids Res. 19: 43-51
5. Staudt, L.M., and Lenardo, M.J. (1991) Ann. Rev.
Immunol. 9: 373-396
6. Latchman, D.S. (1996) Int. J. Biochem. Cell Biol.
28: 1081-1083.
7. Strublin, M., et al. (1995) Cell 80: 497-506
8. Tanaka, M., et al. (1992) Cell 68: 755-767.
9. Lillycrop, K.A. and Latchman, D.S. (1992) J.
Biol. Chem. 267: 24960-24965.
10. Kendall, G., et al. (1995) J. Neurosci Res. 40:
11. Lai, J.-S. & Herr, W. (1992) Proc. Natl. Acad. Sci.
USA 89: 6958-6962.
12. Scheidereit, C., et al. (1987). Cell 51: 783-793.
13. Clerc, R.G., et al. (1988) Genes Dev. 2: 1570-
NOTES: Oct-2 can be detected in 50 - 100 mg of Daudi cell lysate by western
blotting with NA44. Other
cell lines known to express Oct-2 include WEHI231, BJA-B, HAFTL 1, and
X63Ag8 (4,12,13). HeLa and
293 cells (11) do not express detectable levels of Oct-2 protein. The
predominant band detected is 55 - 60
kDa which may appear as a doublet due to expression of additional isoforms.
A second band of approx. 75
kDa, corresponding to the Oct2-5/Oct-2b isoform (4,11), is also detected,
albeit at reduced amounts with
NA44 but not with NA45. Careful titration of the secondary detector antibody
may be necessary for
optimum signal.

-----Original Message-----
From: Drew Sally A. []
Sent: Thursday, February 21, 2002 1:16 PM
To: 'Histonet'
Subject: Oct-2 Antibody?

Are any of you wonderful people familiar with an antibody called Oct-2?  We
have a hematopathologist asking us what it is...

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