RE: Lousy tissues
For routine processing, I would suggest ditching at least one of the wax
stations, if not two, and at least one or two of formalin.
At my facility, we use 3 100% alcohols to ensure proper dehydration, and 2
xylenes for proper clearing.
If you don't dehydrate and clear effectively, 40 wax baths won't give proper
Are the tissues unfixed, or un-infiltrated (is that a word?)
My guess is that the infiltration is the culprit.
Northwestern Medical Center
St Albans, VT 05478
From: Walzer Susan [mailto:Susan.Walzer@HCAHealthcare.com]
Sent: Tuesday, February 26, 2002 10:34 AM
To: 'lynn brennan'; email@example.com
Subject: RE: Lousy tissues
Are you changing solutions regularly? This is escpecially important if you
have a high volume of work.
From: lynn brennan [mailto:firstname.lastname@example.org]
Sent: Friday, February 22, 2002 2:51 PM
Subject: Lousy tissues
HELP, help. Our tissues are coming out unfixed, not easy to cut, and we need
Could anyone suggest something. We use allegiance 10% formalin. Shanndon
Processor. We spend 1 hour in each station. 3 formalins, 70%, 2 95%, 2
2 xylenes, 4 Paraffin.
Any help would be appreciated.
City of Hope Med Center
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