Maria,
I make up 20X Tris and then make it up weekly, wouldn't let it sit much
longer.

Mary Georger
Center for Cardiovascular Research
University of Rochester Medical Center
KMRB Room 2-9816
601 Elmwood Avenue.
PO Box 679
Rochester, New York  14642
585-273-1548

> ----------
> From: 	Maria Mercier
> Sent: 	Wednesday, February 20, 2002 4:05 AM
> To: 	histonet@pathology.swmed.edu
> Subject: 	Immuno Buffers
> 
> Hi all
> 
> Can one make up Tris and EDTA buffers in bulk and use
> as needed or is it better to make them up fresh?At
> what temp should they be kept and what is their shelf
> life?
> 
> Thanks in advance for your help.
> 
> Maria
> 
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