Diane,

The dye you require is Sirius Red 4B(C.I. 28160) also known as Direct Red 81.
It is listed in the Sigma-Aldrich Handbook of Stains etc. p274
Regards

Bryan

----------
From:   Boneslides@aol.com[SMTP:Boneslides@aol.com]
Sent:   February 21, 2002 11:00 AM
To:     histonet@pathology.swmed.edu
Subject:        HBQ Stain

Hello Histonetters:
    My pathologist returned from a meeting with a request for an HBQ stain
(Hall's and Brunt's Quadruple stain).  One of the components is Direct Red
(C.I. 28160).  I have done a little research and have discovered that Congo
Red is also called Direct Red Y, R and C, but the C.I. # is different.  Sigma
only lists Congo Red in their catalogue and I couldn't locate Direct Red in
any of my other usual sources.  Can anyone recommend another distributor that
I might try?  Also, since the procedure calls for a 0.5% aqueous Direct Red
solution (appears to be a counterstain), what are the chances that
substituting Congo Red will work?
    Any input will be greatly appreciated!

Thanks,
Diane

Diane Mahovlic, HT(ASCP)
Orthopedic Pathology and Biomaterials Laboratory
The Cleveland Clinic Foundation
Cleveland, Ohio

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