|From:||Hewlett Bryan |
The dye you require is Sirius Red 4B(C.I. 28160) also known as Direct Red 81.
It is listed in the Sigma-Aldrich Handbook of Stains etc. p274
Sent: February 21, 2002 11:00 AM
Subject: HBQ Stain
My pathologist returned from a meeting with a request for an HBQ stain
(Hall's and Brunt's Quadruple stain). One of the components is Direct Red
(C.I. 28160). I have done a little research and have discovered that Congo
Red is also called Direct Red Y, R and C, but the C.I. # is different. Sigma
only lists Congo Red in their catalogue and I couldn't locate Direct Red in
any of my other usual sources. Can anyone recommend another distributor that
I might try? Also, since the procedure calls for a 0.5% aqueous Direct Red
solution (appears to be a counterstain), what are the chances that
substituting Congo Red will work?
Any input will be greatly appreciated!
Diane Mahovlic, HT(ASCP)
Orthopedic Pathology and Biomaterials Laboratory
The Cleveland Clinic Foundation
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