RE: Fixatives

From:Jasmine Piep

Is your cells in a coated chamber slides, in a Tissue culture dish or on a 
glass coverslip? If it's in a glass coverslip, make sure they're coated 
slides and not a normal glass coverslip.

Jasmine

>From: "Georger, Mary" 
>To: histonet@pathology.swmed.edu, 'Sylvia Poulos' 
>
>Subject: RE: Fixatives
>Date: Fri, 15 Feb 2002 15:43:56 -0500
>
>Sylvia,
>We fix our cell in Methanol/Acetone !:! for 10 minutes at -20, then rinse
>twitce in PBS, then begin our Immunos. If you are losing cells perhaps they
>have dried at some point?
>Best of luck!
>
>Mary Georger
>Center for Cardiovascular Research
>University of Rochester Medical Center
>KMRB Room 2-9816
>601 Elmwood Avenue.
>PO Box 679
>Rochester, New York  14642
>585-273-1548
>
> > ----------
> > From: 	Sylvia Poulos
> > Sent: 	Friday, February 15, 2002 1:56 PM
> > To: 	histonet@pathology.swmed.edu
> > Subject: 	Fixatives
> >
> > Hi everyone,
> > I need some help from those of you who know about doing work will cell
> > cultures.  Somewhere in the process of immunostaining culture dishes the
> > cells are disappearing.  I was told this may be a fixative problem- I 
>use
> > 4% paraformaldehyde for 30 minutes.  Could it be?  Any other suggestions
> > as to why this is occurring or how to fix it would be greatly 
>appreciated!
> > Thanks,
> > Sylvia Poulos
> > Athens, GA
> >
> >
> >
>




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