Is your cells in a coated chamber slides, in a Tissue culture dish or on a
glass coverslip? If it's in a glass coverslip, make sure they're coated
slides and not a normal glass coverslip.
>From: "Georger, Mary"
>To: firstname.lastname@example.org, 'Sylvia Poulos'
>Subject: RE: Fixatives
>Date: Fri, 15 Feb 2002 15:43:56 -0500
>We fix our cell in Methanol/Acetone !:! for 10 minutes at -20, then rinse
>twitce in PBS, then begin our Immunos. If you are losing cells perhaps they
>have dried at some point?
>Best of luck!
>Center for Cardiovascular Research
>University of Rochester Medical Center
>KMRB Room 2-9816
>601 Elmwood Avenue.
>PO Box 679
>Rochester, New York 14642
> > ----------
> > From: Sylvia Poulos
> > Sent: Friday, February 15, 2002 1:56 PM
> > To: email@example.com
> > Subject: Fixatives
> > Hi everyone,
> > I need some help from those of you who know about doing work will cell
> > cultures. Somewhere in the process of immunostaining culture dishes the
> > cells are disappearing. I was told this may be a fixative problem- I
> > 4% paraformaldehyde for 30 minutes. Could it be? Any other suggestions
> > as to why this is occurring or how to fix it would be greatly
> > Thanks,
> > Sylvia Poulos
> > Athens, GA
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