Our method is:
1. Take sections down to water
2. Put section into potassium permanganate soln for 9 min.
3. Rinse in water
4. Bleach in 5% Oxalic Acid
5. Rinse in water
6. Stain with Highman's Congo Red.
A duplicate slide is stained with the Congo Red only for direct comparison
Permanganate Resistant - primary and multiple myeloma amyloid; senile
Permanganate sensitive - secondary amyloid
Hope this helps!
Tech II - Special Stains
Anatomic Pathology - Foothills Medical Center
Calgary Laboratory Services
Ph - 403-670-4760
Fax - 403-270-4093
From: firstname.lastname@example.org [mailto:email@example.com]
Sent: Friday, February 22, 2002 8:59 AM
One of our Pathologists wants to differentiate between 2 types of Amyloid.
says there is a stain using potassium permanganate and ? Congo Red, but I
not find it in any manual, Help
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