Processing Routine

From:Jeff Silverman

Lynn- You are wasting valuable dehydration space with three formalin
stations. You only need one long fixation bath, just change the fixative
every day or every other day if light. Try this:

Switch to one formalin for three to five hours (we use five hours).
70% alcohol- 40 minutes
100% 40 minutes (this bath will become 95% the first time you use it.)
100% 40 minutes
100% 40 minutes
100% 40 minutes
100% 40 minutes
100% 40 minutes
Xylene 1 hour
Xylene 1 hour
Wax 40 minutes
Wax 40 minutes
Wax 40 minutes
Wax 40 minutes

Works very well for us using Histochoice fixative, pure ethanol from
pharmacy, xylene, and plain Paraplast, which same we use also to embed in.
Hope this helps.

Jeff Silverman HT HTL QIHC (ASCP)
Southside Hospital
Bay Shore NY USA





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