Osmium Tetroxide

From:Anila Syed

Hi all,
Apologies if this has been asked before.
My question is:
I am embedding corpus callosum in epoxy resin in order to quantify the fibres. I will stain with Toluidine Blue.
I am going to fix in glutaraldehyde overnight at 4 deg C. But would I also need to use osmium tetroxide? My reasoning is that it will help to stabilise the lipids in the myelin sheaths and therefore give me a much clearer picture of what is going on.

This is only for LM and will not go to the EM at all.
Many thanks in advance,
Anila Syed

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