Odds and ends replies
We too recently had a cranial bone specimen with a metaplastic menigioma
diffusely infiltrating the bone. There was no EMA or vimentin
immunoreactivity after Cal-Rite decal for three days and post fixation in
Histo Choice.
Mast cell granules also express CD68 (KP1), a component of lysosomal
membrane.
We run a Leica TP 1050 processor and so far it has been fine. We also use
the Leica XL stainer and love it.
Make your own RDO- 15 % HCl and add some activated charcoal for authenticity
:-)
Fat control- fat, breast, etc are too hard to cut well and you get a semi-
smear rather than a good section. The ideal fat control is a normal adrenal-
the lipids in the adrenal cortex section adn stay localized much better than
greasy adipose tissue. The capsule and medulla should be devoid of fat. I
remember borrowing an adrenal from the medical examiner's office for my HT
exam back in 1972.
Artefact-- Looks like some weird iron pigment (hematoidin??) in the
placental Hofbauer cells (macrophages :-).
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