Mineralised Bone biopsies

From:Andrew Kennedy

Hi Histonetters,

We are in the process of setting up a Mineralised Bone Histo section in our
Department and we need some advice. We are continuing the work of another
department who have handed over this job to us and would like to streamline
the process a bit. The way they make up their resin is long, tedious and
tricky, so that is the first thing I would like to "fix". Secondly we use
"spare time" on another departments' old sledge microtome to cut the bones
and I would like to purchase our own equipment to get away from having to
fit in with another lab.
So firstly, has anyone had any experience cutting Mineralised (not
decalcified!) bone on a heavy duty rotary microtome or should we be using a
Secondly, what resins do you use and are they available in easy to use kit

Thanks for any help in advance

Andrew Kennedy

Senior Science Officer in charge - Histopathology.
Department of Anatomical Pathology
Concord Repatriation General Hospital
Hospital Road
Concord, NSW, Australia
Phone: +612 9767 6115
Fax: +612 9767 8427

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