Journal of Histochemistry and Cytochemistry correct website
Journal of Histochemistry and Cytochemistry website is WWW.JHC.org, sorry
about that! Hmmm wonder what the flower web page is like???? Off to
Internet!
At 03:07 PM 2/27/02 -0600, you wrote:
>Hi Gayle,
> The address you gave sends you to a flower web page. Just thought I
>'d let you know. Jenny
>
>-----Original Message-----
>From: Gayle Callis [mailto:uvsgc@montana.edu]
>Sent: Wednesday, February 27, 2002 12:24 PM
>To: Brandon Schanbacher; histonet@pathology.swmed.edu
>Subject: Re: Texas Red and RBCs
>
>
>The March, 2002 J of Histochemistry and Cytochemistry had a wonderful
>article on quenching autoflourescence. Go to www.JHC.com and look it up.
>
>At 11:48 AM 2/27/02 -0500, you wrote:
>>I need some help with an autofluorescence problem. I'm using immersion
>>fixed FFPE rat cardiac tissue and have been trying a Texas Red
>>conjugated secondary antibody. When I look at the tissues using either
>>a Texas Red or a WG filter cube, the red blood cells light up like
>>christmas. I'm using a connexin antibody, so the RBCs should not be
>>positive. The fluorescence doesn't appear to be due to antibody
>>nonspecificity as I've seen this in unstained tissues as well. I've
>>tried to quench the autofluorescence with NaBH4 (0.1% in PBS), but to no
>>avail. I'm not sure what else to try and am ready to switch to another
>>fluor, but would really like to know what is going on here. Has anyone
>>else had similar problems. Is there a way to correct this, or is Texas
>>Red just not compatable with bloody tissues?
>>
>>
>>Thanks,
>>
>>Brandon Schanbacher
>>--
>>Division of Pharmacology
>>Ohio State University
>>
>>
>>
>>
>>
>Gayle Callis
>MT,HT,HTL(ASCP)
>Histopathology Supervisor
>Veterinary Molecular Biology - Marsh Lab
>Montana State University - Bozeman
>19th and Lincoln St
>Bozeman MT 59717-3610
>
>406 994-6367
>406 994-4303 (FAX)
>
>
>
Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
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