Journal of Histochemistry and Cytochemistry correct website

From:Gayle Callis

Journal of Histochemistry and Cytochemistry website is, sorry
about that!  Hmmm wonder what the flower web page is like????  Off to

At 03:07 PM 2/27/02 -0600, you wrote:
>Hi Gayle,
>	The address you gave sends you to a flower web page. Just thought I
>'d let you know. Jenny
>-----Original Message-----
>From: Gayle Callis []
>Sent: Wednesday, February 27, 2002 12:24 PM
>To: Brandon Schanbacher;
>Subject: Re: Texas Red and RBCs
>The March, 2002 J of Histochemistry and Cytochemistry had a wonderful
>article on quenching autoflourescence.  Go to and look it up.  
>At 11:48 AM 2/27/02 -0500, you wrote:
>>I need some help with an autofluorescence problem.  I'm using immersion
>>fixed FFPE rat cardiac tissue and have been trying a Texas Red
>>conjugated secondary antibody.  When I look at the tissues using either
>>a Texas Red or a WG filter cube, the red blood cells light up like
>>christmas.  I'm using a connexin antibody, so the RBCs should not be
>>positive.  The fluorescence doesn't appear to be due to antibody
>>nonspecificity as I've seen this in unstained tissues as well.  I've
>>tried to quench the autofluorescence with NaBH4 (0.1% in PBS), but to no
>>avail.  I'm not sure what else to try and am ready to switch to another
>>fluor, but would really like to know what is going on here.  Has anyone
>>else had similar problems.  Is there a way to correct this, or is Texas
>>Red just not compatable with bloody tissues?
>>Brandon Schanbacher
>>Division of Pharmacology
>>Ohio State University
>Gayle Callis
>Histopathology Supervisor
>Veterinary Molecular Biology - Marsh Lab
>Montana State University - Bozeman
>19th and Lincoln St
>Bozeman MT 59717-3610
>406 994-6367
>406 994-4303 (FAX)
Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)

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