IHC pictures

From:rueggp

Dear Friends,

Now that I am retired I need your help accessing some material for a
workshop I will be presenting.

I am putting together a workshop on "Taking the QIHC Exam" and I need
some pictures of IHC stains required for the exam.  I have many already
from taking the exam myself on the first go around.  I was wondering if
there are web sites posting microphotographs of IHC and ISH stained
slides.  We have several pictures on our web site www.ihcrg.org but I
would like to have some more.  If you know of an internet resource for
pictures I would appreciate having that info.  If you have glass slides
of these stains I can make the pictures and send you back the slides.

Here is a list of some of the pictures I need:

IHC Chromogranin         a.  1 slide
                                      b.  tissue - stomach
                                      c.  paraffin embedded

Antigen Titration                  a.  3 slides representing 3 different

Factor VIII Related Antigen      dilutions between 1:5 and 1:160,
                                                one which is optimal
                                            b.  tissue - Hodgkin's
lymphoma
                                            c.  paraffin embedded

IHC                                 a.  3 slides
Control Selection CEA     1.  a carcinoma which does NOT express CEA-
                                             labeled "Patient"

                                         2.  an appropriate control to
exclude the possibility that
                                              the absence of
immunoreactivity is the result of poor
                                              procedure-labeled
"Control"
                                         3.  an appropriate control to
exclude the possibility that
                                              the absence of
immunoreactivity is NOT due to the
                                              loss of antigenicity in
the tissue block-labeled
                                              "Antigen Control"

S-100                                a. one slide of skin

Thank you for your help.
Patsy Ruegg





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