Tree Shrews are mammals closely related to
primates. (Tupia Glis) The protein sequences that we have are very
similar (95 % or more) to human. We are one of 3 labs in the US breeding
them.
We haven't done any IHC to speak of but were thinking of doing some
frozen sections of Sclera in the future. I don't really know the
specifics of fixation but Pam tried some sections that were fixed. I've
been trying comercial antibodies (human antibodies known to work in other
species) in western blots. An ideal antibody could be used for either
technique. Proteins of interest are extracellular matrix proteins (TIMP I
& II, MMP 1-3, 26, more...). We know these proteins are in sclera and
expressed at detectable levels. I feel that my extraction and western
blotting techniques are working. I had success with a human Actin
antibody and with a TIMP 2 human polyclonal that is no longer avalable.
JOEL
Tammy M. Bailey
University of Alabama Birmingham
Histology Module
Vision Science Research Center
205-934-3074-lab
205-410-8029-cell
tbailey@uab.edu
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