Does anyone out there have any tricks on how to
prevent/minimize freezing artefact when freezing brain biopsy tissue? We are
currently using Isopentane/Liquid Nitrogen. Have also tried freezing tissue on
our new Leica cryostat using the Pelletier element. Both methods have resulted
in varying degrees of success and failure. Also, we use Gum Tracacanth as our
freezing medium (because that's what's always been used here). Any
thoughts on this would be more than welcome.
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