DAB and beta-galactosidase

From:Anne Gysembergh

Hi everyone!
I need your experience as I am totally discovering your world HISTOLOGY.
In immunoassays, I know that the most common substrate for
beta-galactosidase is BCIG (bromochloroindolyl-beta-D-galactopyranoside).
However, I recently did a control slide with tissue I knew there was
endogenous beta-gal activity.
Steps were the following:
- No primary antibody
- Biotinylated secondary antibody
- Avidin-HRPO
I obtained a brownish color where I know there is beta-gal protein. Is it
Any comment of advice will be very greatly appreciated.
Thank you
Anne Gysembergh, PhD
INSERM U523 - Gene Therapy Lab
Institut de Myologie
Bâtiment Babinski
Groupe Hospitalier de la Pitié-Salpétrière
47, Boulevard de l'Hôpital
75651 Paris Cédex 13
Phone: (33) (0)1-42-16-57-12
Fax: (33) (0)1-42-16-57-00
mailto: a.gysembergh@myologie.chups.jussieu.fr

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