Cytoprep

From:Janice Fuller

Histonetters,
 
I have a few questions:  Is albumin used in the cytospin for any other reason than adhesion?  What about if your already using plus or coated slides?  I've heard for a long time that if you use an adhesive in your waterbath and then use plus slides it changes the charge and tissue falls off.  I've not experienced this personally, but it seems that it would be as true for cytology as histology.
 
Also, what about air drying cytospin slides for a Giemsa stain.  Shouldn't this be a Wright Giemsa?  Why would this be preferable to fixing in 95% Alcohol and pap staining?   I'm trying to get a feel for the best way to set up a lab for cytoprep.
 

Janice Fuller
DeWine@compaq.net

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