I have a pathologist who would like me to order Cyclin E. I have checked my usual sources and can't seem to find it. Anyone out there use this antibody?
Also, the hospital I am at now has a Ventana Nexus immunostainer. It is not uncommon for us to have problems every couple of weeks or so with this little gem. ;>)) It sounds like a lot of you do your immunos by hand. Is this correct? I used to do them by hand, used concentrated antibodies, etc. I inherited this stainer when I started at this job a year ago. I am currently working my way back to concentrated antibodies, rather than the Ventana predilutes, and at times toy with the idea of doing them by hand again. One advantage that I see with the Nexus is the use of heat. But when it goes down I am down 3-5 days. This does not make for happy pathologists! Any comments are welcome!
Hillcrest Medical Center
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