thermonox coverslips -Reply
|From:||Tony Henwood <AnthonyH@chw.edu.au>|
I would suggest fixing the coverslips in 95% ethanol (minimum 20min)
and then doing your IPX on them. Most antibodies work very well on
ethanol fixed material. There is no antigen retieval required (no formalin
or other cross-linking fixatives used). S100, though can be problematical.
The antigen is somewhat alcohol soluble, so in some cases a false
negative result will occur.
Hope this helps.
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318
>>> "CRME (Criss Meligro)" <email@example.com> 22/February/2001
Have been asked to prepare cells grown on themonox coverslips for
stains including immunohistochemistry.....(in paraffin??)
I have not worked with this.... any suggestions? THANKS in advance!
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