thermonox coverslips -Reply

From:Tony Henwood <>

Dear Criss,
I would suggest fixing the coverslips in 95% ethanol (minimum 20min)
and then doing your IPX on them. Most antibodies work very well on
ethanol fixed material. There is no antigen retieval required (no formalin
or other cross-linking fixatives used). S100, though can be problematical.
The antigen is somewhat alcohol soluble, so in some cases a false
negative result will occur.
Hope this helps.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

>>> "CRME (Criss Meligro)" <> 22/February/2001
06:54am >>>
Have been asked to prepare cells grown on themonox coverslips for
stains including immunohistochemistry.....(in paraffin??)
I have not worked with this.... any suggestions?    THANKS in advance!  

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