neonate mouse bones

From:Karen Glyde <>

Hello all,  I need to do  IHC on neonatal mouse bone days 4,13 and 18.  The
particular interest is in the  epiphyseal plate of the tibia
(chondrocytes)  The info I have rouned up suggests acid decalcification is
not appropriate   when followed by histochemical techniques and
investigations but rather the use of citrate buffer (pH4.5) after a 24 to
48 hour fix in 80% cold alcohol.  OR is bone this young still cartilaginous
enough not to need decalcification?   Reactions? Suggestions?  Has someone
done this?

Karen L.Glyde
Electron Microscope Facility
Life Sciences Consortium
Pennsylvania State University

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