bone sections
From: | "Renton, Lousie, Mrs" <177louie@chiron.wits.ac.za> |
Hi there, especially all you boneheads!
I am looking for a better way to mount our MMA sections.
The protocol that we follow is:
6um sections are cut and stored in 30% alcohol. They are then
stained freefloating, and placed in absolute alcohol. We mount
them from abs alc in Entellan, and allow them to dry overnight. I
find that the entellan tends to show a lot of shrinkage, and that the
miscibility of alcohol and entellan is not optimal. Are there
alternaitve methods that other workers are willing to share that
might be an improvement?
I subscribe to the maxim"if it aint broke, don't fix it" but
sometimes......
best regards
Louise
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