bone sections

From:"Renton, Lousie, Mrs" <177louie@chiron.wits.ac.za>

Hi there, especially all you boneheads!

I am looking for a better way to mount our MMA sections.
The protocol that we follow is:
6um sections are cut and stored in 30% alcohol. They are then 
stained freefloating, and placed in absolute alcohol. We mount 
them from abs alc in Entellan, and allow them to dry overnight. I 
find that the entellan tends to show a lot of shrinkage, and that the 
miscibility of alcohol and entellan is not optimal. Are there 
alternaitve methods that other workers are willing to share that 
might be an improvement?
I subscribe to the maxim"if it aint broke, don't fix it" but 
sometimes......

best regards
Louise 




<< Previous Message | Next Message >>