From:Kenneth Wester <>

Hi All.

Is there anyone who can give me informatiom on how to properly use the
Zamboni-fixative on cryo-sections? In my hands, the immunoreactivity is
preserved excellently, e.g., for EGF-receptor staining, after Zamboni
fiaxation. However, the overall morphology is poor, even when compared to
fixation in aceton and/or methanol.

I have tested air-drying the sections for between 30 seconds up to 1 hour,
without any major improvement. Usually I fix for 2-3 minutes followed by
rinsing in distilled water.

Greatful for any tips regarding the morphology problem,

Kenneth Wester

Kenneth Wester, Ph.D.
Depts. of Surgical Sciences and Genetics & Pathology
Experimental urology, The Rudbeck laboratory
Uppsala University
Dag Hammarskjöldsväg 20
S-752 37 Uppsala, Sweden
phone:  +46 (0)18 611 02 06
fax:    +46 (0)18 471 3432

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